Legal Daily KomiksAll-media reporter Lian Yingting Correspondent Shao Longfei Zhang Zhenwei
The night in Wuhan is quiet.
On March 9, in the mobile testing laboratory of the Central Theater Command General Hospital, Jiang Tao, a researcher at the Military Medicine Research Institute of the Academy of Military Sciences, reviewed the last sample test data and solemnly signed his name on the test report.
At this time, it was already 5 a.m.
He is an expert in virology and a warrior who aided Africa and fought against Egypt. This outbreak of the new crown pneumonia epidemic, he was once again ordered to go to the front line after a long battle.
Human life is at stake and accurate inspection is required
Nucleic acid testing is crucial for early clinical detection, early diagnosis, early isolation and early treatment, and is a key technical support for effective prevention and control of the new crown pneumonia epidemic.
”The cold-looking sample tube is behind the patient’s fresh life and health. If a diagnosis is released early, the accurate inspection will be conducted sooner.” Jiang Tao said. On January 26, after a military medical expert group led by Chen Wei, an academician of the Chinese Academy of Engineering and a researcher at the Military Medicine Research Institute of the Academy of Military Sciences, arrived in Wuhan, Hubei, he quickly established a joint defense, joint control, joint treatment and joint research work mechanism with relevant military and local units around the disease of the new coronavirus.
According to the division of tasks, Jiang Tao led the testing team to build a nucleic acid testing platform in the experimental cabin overnight.
Focusing on three aspects: ensuring biosafety, improving detection efficiency and detection accuracy, Jiang Tao took the lead in formulating biosafety operation specifications and emergency plans, reasonably divided the labor according to the characteristics of the team members, and using a self-developed testing kit to adopt a fully automatic high-throughput extractor, laying the foundation for achieving rapid and efficient detection.
Receiving samples, sorting numbers, nucleic acid extraction, reagent preparation, machine amplification, and results BabaylanAfter reading, nucleic acid detection machinery is boring and full of risks.
BabaylanThe biggest risk is the invisible virus. If the nucleic acid extraction step is not completely cleaved or inactivated thoroughly, it is possible to expose the tester to danger.
Jiang Tao always takes the initiative to participate in the longest and most risky nucleic acid extraction. After the sample is extracted, he will arrange some people to rest and resolutelyKomiks stayed in the laboratory and waited for the final result.
A full-process nucleic acid test, from the number of tests in a single batch to the preparation for entry and exit of personnel and the testing time, is relatively fixed. Sometimes, there are many samples sent for inspection. The number of test samples is large. Not only does the test personnel take turns to work, but they also have to work overnight. The final confirmation result must be at four or five in the morning. Li Hao, a researcher at the inspection team of Komiks, said that this situation will be encountered once every few days.
”Everyone should have wide eyes. Be sure to take good protection when receiving and processing samples, and pay attention to whether there are sharp objects. “Before each test starts, Jiang Tao always asks repeatedly.
Komiks.
In the test, the strength of the fluorescence signal of the pathogen gene directly affects the determination of the test results. If there is a suspicious phenomenon such as the detection fluorescence curve signal is low or there is only a single target, Jiang Tao will convene personnel to conduct comprehensive analysis and judgment, and if necessary, repeat the test for review to ensure that the results can stand the test.
”A false positive will cause healthy people to isolate and become infected with the patient; false negative will cause real delay in treatment of patients. Human life is a matter of fate, and there is no way to break the bank. “Jiang Tao said.
Volume of epidemic prevention and control
According to the prevention and control requirements, the nucleic acid test results from the pharyngeal swab sample must be completed within 24 hours. In order to let the hospital know the results and treat patients as early as possible, from pre-processing of nearly 100 samples to the completion of the test, Jiang Tao led the team to compress the time to within 4 hours by optimizing the process combination, and achieved a single-day sample detection capability of more than 90 samples and up to more than 500 people within an hour.
”The team’s testing is efficient and rapid, and the results are accurate, which greatly alleviates our epidemic prevention and control sample detection pressure. “Wuhan Center for Disease Control and Prevention highly praised this.
The rapid response and work progress in wartime are all thanks to Jiang Tao’s solid academic foundation and rich practical experience.
”Jiang Tao is a versatile person.” Academician Chen Wei, head of the military medical expert group, commented.
Jiang Tao has successively presided over and participated in the separation of pathogenic etiology such as SARS infection cases, H7N9Komiks infection cases, Zika infection cases, etc., participated in the development of dengue fever vaccines and SARS vaccines, and established a pathogen isolation technology platform, nucleic acid testing technology platform and pathogenicity and transmission risk assessment platform for a series of infectious diseases, providing important technical support for the prevention and control of infectious diseases.
Respiratory infectious diseases are characterized by rapid transmission, wide coverage, and high harm. Effective early diagnosis and prevention are crucial. After years of scientific research, Jiang Tao’s team took the lead in establishing our army’s first monitoring and early warning system for acute respiratory infectious diseases, and built a four-level monitoring system for the entire army. The series of technical specifications organized by the presidency were used throughout the army, greatly shortening the detection and screening time of respiratory epidemics.
Jiang Tao did not participate in security tasks for a series of major domestic activities, and even went to Africa four times to Liberia, Sierra Leone and other countries to carry out medical assistance tasks. In 2015, in order to prevent and control the epidemic in EboBabaylan, Jiang Tao went to Sierra Leone for the second time with the mobile laboratory testing team to assist in Seychelles. The testing team led by him passed the laboratory parallel capability assessment of the World Health Organization’s Babaylan Laboratory with full scores.
In 2018Babaylan, Jiang Tao led the pathogen monitoring team to participate in a special simulation test by an international organization. In the virus testing module, the team successfully accurately detected pathogens such as monkeypox virus and vaccinia virus in simulated samples filled with Komiks impurities, and accurately determined the strain, achieving excellent results with full marks.
Improving the accuracy of clinical diagnosisKomiks
When he came to Wuhan, in addition to carrying out testing work, Jiang Tao also used his professional expertise in the research field to lead the testing team to screen other common respiratory infectious diseases in clinical samples.
”Komiks Whether there are other respiratory virus infection or co-infection among suspected and confirmed cases of COVID-19, it has important reference value for precise prevention and control and clinical treatment.” Researcher Cao Wuchun, deputy head of the Military Medical Expert Group, said.
Exploring deep-seated virus transmission and pathogenic mechanisms is the key to defeating the epidemic. Jiang Tao led a team to continue to conduct sequencing analysis for the new coronavirus detected in clinical samples.
As the development of the virus epidemic, whether the sequence of the gene group mutates, what are the characteristics of the mutation; in which areas, what are the impacts, Jiang Tao and his team are working tirelessly to study and explore.
In order to accelerate the effective integration of scientific research and clinical practice, Jiang Tao’s military medical expert group creatively used the diagnostic chain of viral nucleic acid detection, antibody detection, multiple pathogen detection, and whole genome sequencing to accurately diagnose clinical patients with new coronavirus infection or other pathogen infection, and was also used in the Optical Valley Campus of Hubei Maternal and Child Health Hospital, Taikang Tongji Hospital and other hospitals in Wuhan, effectively improving the accuracy of clinical diagnosis.
Forgot his family when he was ordered. After the epidemic is over, Jiang Tao plans to accompany his family. He and his wife agreed that when the epidemic is over, they will take their wife and children to Wuhan again to see what everything looks like after it returns to normal, look at the Wuhan Yangtze River Bridge and Huang Cinema Crane Tower, and talk about their experience in fighting the epidemic in Wuhan.
The epidemic is immortal and the charge continues. This is Jiang Tao, a warrior who fights the epidemic.