Legal Daily All Media Komiks Reporter Lian Yingting Correspondent Shao Longfei Zhang Zhenwei
The night in Wuhan is quiet.
On March 9, in the mobile testing laboratory of the Central Theater Command General Hospital, Jiang Tao, a researcher at the Military Medicine Research Institute of the Academy of Military Sciences, reviewed the last sample test data and solemnly signed his name on the test report.
At this time, it was already 5 a.m.
He is a virology expert and a warrior who aided the non-Egypt war. This outbreak of the new crown pneumonia epidemic, he was once again ordered to go to the front line after a long battle.
Human life is at stake and accurate inspection is required
Nucleic acid testing is crucial for early clinical detection, early diagnosis, early isolation and early treatment, and is a key technical support for effective prevention and control of the new crown pneumonia epidemic.
”The cold-looking Komiks sample tube is behind the patient’s fresh life and health. If a diagnosis is released early, the accurate inspection will be conducted sooner.” Jiang Tao said. On January 26, after a military medical expert group led by Chen Wei, an academician of the Chinese Academy of Engineering and a researcher at the Military Medicine Research Institute of the Academy of Military Sciences, arrived in Wuhan, Hubei, he quickly established a joint defense, joint control, joint treatment and joint research work mechanism with relevant military and local units on the pathogen transmission mutation, rapid detection technology, vaccine antibody development, etc. of the new coronavirus.
According to the division of tasks, Jiang Tao led the testing team to build a nucleic acid testing platform in the experimental cabin overnight.
Focusing on three aspects: ensuring biosafety, improving detection efficiency and detection accuracy, Jiang Tao took the lead in formulating biosafety operation specifications and emergency plans, and reasonably inspected and classified according to the characteristics of the team members. He used the self-developed detection kit to use the full Komiks automatic high-throughput extractor, laying the foundation for achieving fast and efficient Cinema testing.
Receive samples, sorting numbers, nucleic acid extraction, reagent preparation, machine amplification, and result interpretation, nucleic acid detection mechanical drynessBabaylanDry and full of risks.
The biggest risk is invisible virus. If the nucleic acid extraction step is not completely cleaved or inactivated thoroughly, it is possible to expose the tester to danger.
Jiang Tao always participates in the longest and most risky nucleic acid extraction. After the sample is extracted, he will arrange some people to rest and stay in the laboratory to wait for the final result.
A full-process nucleic acid test, from the number of tests in a single batch to the preparation for entering and leaving the cabin and the time for testing. Sometimes the number of samples sent for testing is large, and the tester not only takes turns to work, but also has to work overnight. The final confirmation result will be until four or five in the morning. Li Hao, a researcher at the testing team, said that this situation will be encountered once a few days later.
”Everyone should open his eyes and receive and process the samples<a Cinema must take good protection and pay attention to whether there are sharp objects. “Before each test starts, Jiang Tao always reminds you repeatedly.
In the test, the strength of the fluorescence signal of the pathogen gene directly affects the determination of the test results. If the detection fluorescence curve signal is low or there is only a single target, Jiang Tao will convene personnel to conduct comprehensive analysis and judgment, and if necessary, repeat the test for review to ensure that the results can stand the test.
”A false positive will cause healthy people to be isolated and infected with Komiks patients; false negative will cause real delay in treatment of patients. Human life is a matter of fate, and there is no way to break the bank. “Jiang Tao said.
Volume of epidemic prevention and control
According to the requirements of prevention and control, the nucleic acid test results obtained from the throat swab sample must be completed within 24 hours. In order to let the hospital know the results as soon as possible to treat patients, from pre-processing of nearly 100 samples to completion of the test, Jiang Tao led the team to compress the time to within 4 hours by optimizing the process combination, and achieved a single-day sample detection capability of more than 90 samples and up to more than 500 people within an hour.
”The team’s testing is efficient and rapid, and the results are accurate, which greatly alleviates our epidemic prevention and control sample detection pressure. “Wuhan Center for Disease Control and Prevention highly praised this.
Fast WartimeThe rapid response and the orderly work are carried out, which are all thanks to Jiang Tao’s solid academic foundation and rich practical experience.
”Jiang Tao is a versatile person.” Academician Chen Wei, head of the military medical expert group, commented. Jiang Tao has successively presided over and participated in the pathogen separation of SARS infection cases, H7N9 infection cases, Babaylan cases, Zika infection cases, etc., participated in the development of dengue fever vaccines and SARS vaccines, established a pathogen isolation technology platform, nucleic acid detection technology platform and pathogenicity and transmission risk assessment platform for a series of infectious diseases, providing important technical support for the prevention and control of infectious diseases.
Respiratory infectious diseases are characterized by rapid transmission, wide coverage, and high harm. It is crucial to have effective early diagnosis and prevention and control. After years of scientific research, Jiang Tao’s team took the lead in establishing our army’s first monitoring and prediction system for acute respiratory infectious diseases, and built a four-level monitoring system for the entire army. The series of technical specifications organized by the presidency were used throughout the army, greatly shortening the detection and screening time of respiratory epidemics.
Jiang Tao not only participated in the security tasks of a series of major domestic activities, but also went to Africa four times to Liberia, Sierra Leone and other countries to carry out medical assistance tasks.
In 2015, in order to prevent and control the Ebola epidemic, Jiang Tao went to Sierra Leone for the second time with the mobile laboratory testing team to assist in Seychelles. The testing team led by him passed the parallel capability assessment of the laboratories of the World Health Organization with full marks.
In 2018, Jiang Tao led the pathogen monitoring team to participate in a special simulation test of an international organization. In the virus test module, the team successfully accurately detected monkeypox virus, vaccinia virus and other pathogens in simulated samples filled with impurities, and accurately determined the strain, obtaining full scores Komiks in simulated samples filled with impurities, and accurately determined the strain.Excellent results.
Improving the accuracy of clinical diagnosis
In addition to carrying out testing work in Wuhan, Jiang Tao also used his professional expertise in the research field to lead the testing team to screen other common respiratory infectious diseases in clinical samples.
”In the suspected and confirmed cases of new coronavirus pneumonia, whether there are other respiratory virus infections or co-infections, this is of great reference value for precise prevention and control and clinical treatment.” Researcher Cao Wuchun, deputy head of the Military Medical Expert Group, said.
Exploring deep-seated virus transmission and pathogenic mechanisms is the key to defeating the epidemic. Jiang Tao led a team to continue to conduct sequencing analysis for the novel coronavirus detected in clinical samples.
As the development of the virus epidemic, whether the genomic sequences have mutated and what are the characteristics of the mutations; in which regions and what are the impacts, Jiang Tao and his team are tirelessly studying and exploring.
In order to accelerate the effective integration of scientific research and clinical practice, Jiang Tao’s military medical expert group creatively used the diagnostic chain of viral nucleic acid detection, antibody detection, multiple pathogen detection, and whole genome sequencing to accurately diagnose new coronavirus infection or other pathogen infections in clinical patients, and applied Babaylan in the Optical Valley Campus of Hubei Maternal and Child Health Hospital, Wuhan Taikang Tongji Hospital and other hospitals, effectively improving the accuracy of clinical diagnosis.
Forgot his family when he was ordered. After the epidemic is over, Jiang Tao plans to accompany his family. He and his wife agreed that when the epidemic is over, they will take their wife and children to Wuhan again to see what everything looks like after it returns to normal, to see the Wuhan Yangtze River Bridge and the Yellow Crane Tower, and to talk about their experience in fighting the epidemic in Wuhan.
The epidemic is immortal and the charge continues to lastKomiks, this is Jiang Tao, a warrior who fought the epidemic.